| Reagent | Final concentration | Quantity or volume |
|---|---|---|
| DMEM 1 g/L glucose | 438 mL | |
| HI FBS | 10% | 50 mL |
| L-Glutamine | 1% | 5 mL |
| G418 | 700 μg/mL | 7 mL |
| Total | 500 mL |
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