TABLE 2.
Colocalization of p21 with cyclin B1a
p21 | % of cells with indicated cyclin B staining patternb
|
||
---|---|---|---|
Cyt | CN | Nuc | |
Present | 0 | 22 | 6 |
Absent | 38 | 26 | 7 |
To pinpoint the timing of p21 reaccumulation into the nucleus in late stages of the cell cycle, we scored only cyclin B1-positive cells. The data shown are for Hs68 HDF, but similar results were obtained with IMR-90 HDF.
Cyclin B1-specific staining patterns (cf. Fig. 1H) were cytoplasmic (Cyt; late S and early G2 phase), cytoplasmic and nuclear (CN; mid-G2), and predominantly or exclusively nuclear (Nuc; late G2 and early stages of prophase).