FIG. 1.

Probe A3.5 recognizes a transcript down-regulated during differentiation of HT-29-M6 cells. (A) HT-29-M6 cells were preconfluent or cultured to confluence for the indicated periods of time (days [d]); (B) 17-day postconfluent HT-29-M6 cells and 5-day postconfluent Caco-2 cells were treated with 200 nM TPA for the indicated times. Total RNA (12 μg/lane) was isolated and processed and filters hybridized with ³²P-labeled band A3.5 as described in Materials and Methods. Marks to the right of the autoradiograms indicate migration of 28S and 18S rRNAs. C, untreated control cells.