FIG. 10.
Effect of the constitutive expression of UEV-1 on the contact-induced expression of MUC5AC in HT-29-M6 cells. Control (lane pair 1) and independent clones of UEV-1-transfected HT-29-M6 cells (lane pairs 2 to 5) were harvested 2 days after seeding (lanes a) or 10 days after reaching confluence (lanes b). Transfected clones expressed different levels of the exogenous UEV-1, as determined by RT-PCR, the lowest levels corresponding to clone 2. Total RNA was analyzed by Northern blotting with a probe for the apomucin gene MUC5AC.