FIG. 5.

EGF-induced S-phase entry is restored by IP₃ and DAG in cells injected with PLC-γ1 SH2-SH2-SH3 but not in cells injected with Grb2 SH2 or anti-Ras. Quiescent MDCK cells were injected with the proteins indicated at the bottom of the figure, incubated at 37°C for 1 h, incubated with EGF (100 ng/ml) and BrdU with or without DAG for 15 h, and then processed for immunofluorescence. The percent BrdU-positive cells was calculated as described in Materials and Methods. Data are means + standard errors of three independent experiments.