FIG. 6.

Relief of the PLC-γ1 SH2-SH2-SH3 inhibition of EGF-induced S-phase entry by DAG. Quiescent MDCK cells were injected with PLC-γ1 SH2-SH2-SH3, incubated at 37°C for 1 h, and then incubated with EGF (100 ng/ml) and BrdU with or without DAG for 15 h. The cells were fixed and processed for immunofluorescence, and the percentage of BrdU-positive cells was calculated as described in Materials and Methods. Data are means + standard errors of three independent experiments.