FIG. 7.

Microinjection of PLC-γ1 SH2-SH2-SH3 inhibits EGF-induced PKC-dependent, but not PKC-independent, activation of c-fos promoter plasmids. Quiescent MDCK cells were injected with wtSRE-CAT (A to F) or mutant pm18 (G to L) together with PLC-γ1 SH2-SH2-SH3 (C to F and I to L) or GST (A, B, G, and H) and incubated at 37°C for 1 h, incubated with EGF (100 ng/ml) with (E, F, K, and L) or without (A to D and G to J) DAG for 15 h, and then fixed and stained by immunofluorescence. Microinjected cells were identified with an FITC-avidin stain (left panels), and CAT was assayed with polyclonal anti-CAT antibody followed by a TRITC-conjugated antirabbit antibody (right panels). Magnification, ×120.