Figure 1.

CYTH4 is highly expressed in AML cells and inversely correlated with the overall survival of AML patients

(A) After intersecting three datasets, 25 upregulated and 8 downregulated differentially expressed genes (DEGs) were identified.

(B) The significantly upregulated expression of CYTH4 in AML patients compared to healthy donors (HD) (left, GSE67936, ∗p = 0.0116; middle, GSE65409, ∗p = 0.0102; and right, GEPIA2, ∗p < 0.05).

(C) CYTH4 expression is reversely associated with the overall survival of AML patients (left, GEPIA2, p = 0.0014; middle, TIMER, p = 0.0015; and right, UALCAN, p = 0.019). Kaplan-Meier curve was used to analyze the survival distribution of the two groups.

(D) RT-qPCR assay for CYTH4 mRNA in bone marrow mononuclear cells (BMMCs) from AML patients (n = 35) and HD (n = 15). Data are represented as mean ± SD. Statistical significance was assessed by unpaired t test.

(E) Immunoblotting assay for CYTH4 protein in BMMC from AML patients (n = 6) and HD (n = 4).

(F) RT-qPCR assay for CYTH4 mRNA in human AML cell lines (n = 3). The mRNA level of CYTH4 in the mixed BMMCs from five HD was set as 1.0. Data are represented as mean ± SEM. Statistical significance was assessed by ANOVA with Tukey’s multiple comparison test.

(G) Immunoblotting assay for CYTH4 protein expression in human AML cell lines. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.