FIG. 6.

Western blot showing the coimmunoprecipitation of Numb and Nak in embryo extract. Lanes 1, 3, and 5 are embryo extracts prepared from scabrous-GAL4 strains; lanes 2, 4, and 6 are extracts from the cross of scabrous-GAL4 and UAS-myc-nak strains. After immunoprecipitation with anti-c-Myc antibody, lanes 1 and 2 were probed with anti-c-Myc antibody and lanes 5 and 6 were probed with anti-Numb antibody. Control lanes 3 and 4 are embryo extracts before immunoprecipitation and probed with anti-Numb antibody.