FIG. 1.

hILP blocks cell death induced by a variety of stimuli. (A) MCF7F cells were transfected with pEBB (), pEBB-hILP (▪), pCI (□), or pCI-Bcl-x_L (▨). Twenty-four hours after transfection, cells were treated with fresh medium or medium supplemented with either anti-Fas antibody and cycloheximide, rhTNF-α, or cisplatin or were exposed to UV. After 12 h, cells were fixed, stained for β-galactosidase expression, and scored for apoptotic morphology. (B) MCF7F cells were transfected with pCMV-lacZ together with pcDNA3, pcDNA3-caspase 2, or pcDNA3-caspase 8 with or without cotransfection of pEBB-hILP expression vector. At 24 h after transfection, cells were fixed, stained for β-galactosidase expression, and scored for apoptotic morphology. Results are expressed as percent viable cells (number of flat blue cells/number of flat and round blue cells × 100). The data represent means ± standard deviations (n = 3).