FIG. 3.

hILP does not interact with TRAF proteins. 293 cells were cotransfected with the indicated mammalian GST expression vectors, together with expression vectors encoding each of the six indicated TRAF proteins. Lysates were precipitated by incubation with glutathione agarose beads, and TRAF proteins were identified by Western blot analysis as described in Materials and Methods. The expression of the GST chimeric proteins was also confirmed by using an anti-GST antibody (data not shown).