Fig. 5.

Celastrol alleviates ER stress-associated inflammation and oxidative stress via AMPK signaling in diet-induced obesity (DIO) mice. The aortae from DIO and control (Con) treated with saline or celastrol (Cel, 100 μg/kg/day) intraperitoneally (i.p) for 8 weeks were incubated with or without Compound C (CC, 5 μmol/L) for 16 h. A-C The inflammatory markers, including IL-1β, TNF-α, and IL-10 in thoracic aortae tissue, were measured by ELISA; (D) Representative images and quantified dihydroethidium (DHE) fluorescence in mice aortae, Scale bar, 100 μm; (E) Protein expressions of Nox-2 and iNOS in aortae were analyzed by western blotting. Data are expressed as the means ± S.E.M (n = 6/group), ^*P < 0.05, one-way ANOVA followed by Newman-keuls post hoc test