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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: Am J Physiol Cell Physiol. 2005 May 18;289(4):C934–C945. doi: 10.1152/ajpcell.00092.2005

Figure 8. NHE1 activity contributes to mitochondrial Ca2+ accumulation in NHE1+/+ astrocytes.

Figure 8

FCCP/Oligomycin-induced Ca2+ release was determined under [Ca2+]o-free conditions. NHE1+/+ astrocytes were subjected to either 3 h normoxia (CON), or 2 h OGD and 1 h reoxygenation (OGD/REOX). In some experiments, OGD/REOX was performed in the presence of 1 μM HOE 642. Cells were exposed to [Ca2+]o-free buffer containing 10 μM FCCP + 2.5 μg/ml oligomycin for 4 min to stimulate release of Ca2+ from mitochondria. A. Trace showing the representative Ca2+ response in cells following exposure to FCCP/Oligomycin. B. Representative traces of FCCP/Oligomycin-induced Ca2+ release in astrocytes subjected to OGD/REOX in the presence or absence of HOE 642. C. Summary graph of FCCP/Oligomycin-induced Ca2+ release in NHE1+/+ astrocytes under both CON and OGD/REOX conditions and in the absence or presence of HOE 642. Data are means ± SD (n= 60–80 cells, 3 cultures). * p < 0.001 vs. baseline; # p < 0.001 vs. CON; ψ p < 0.001 vs. FCCP/Oligo OGD/REOX.