Figure 3.

Comparison of PCR and SB methods. Subset of 29 species for which presence of a single restriction site within the intergenic spacer (IGS) could be confirmed (see text). a. Relationship between IGS size index estimated from PCR and from Southern Blot hybridization (y = 0.1465x +3.012; r² = 0.0324; P = 0.3498). For reference, dashed line represents equal PCR and SB values. b. IGS size index difference between Southern Blot hybridization and PCR vs. IGS size index based on Southern Blot hybridization. (y = 0.8535x - 3.012; r² = 0.5321; P < 0.0001). Dashed line indicates SB-based index minus PCR-based index = 1 kb.