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Northern blot and real-time quantitative RT-PCR analyses of differentially expressed genes. (A) Northern blot analysis of six randomly selected genes in macrophages. (B) Real-time quantitative RT-PCR analysis of eight cytokine genes. The relative amount of each cDNA level against to TBP cDNA was measured and defined by an arbitrary unit. (10 μg/ml of theophylline treatment approximately corresponds to the clinical plasma level.)
