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[Preprint]. 2025 Sep 8:2025.05.30.657115. Originally published 2025 Jun 3. [Version 2] doi: 10.1101/2025.05.30.657115

Figure 3. Ptbp1 depletion does not induce the astrocyte-to-neuron transition in substantia nigra.

Figure 3.

(A-C) Representative images of the immunostaining results (substantia nigra) of the mouse brains collected at 4 weeks (A), 8 weeks (B), 12 weeks (C) after tamoxifen induction. White arrowheads indicate the locations of astrocytes (tdT+ cells). However, none of the tdT+ cells express either NeuN or TH. The absence of NeuN or TH and tdTomato double positive cells reveals no astrocyte-to-neuron conversion in Ptbp1 cKO. Scale bars are 100 um. Animal numbers are n=3 for both control and KO groups at all the three time points expect for the control group at 8 weeks (n=2).