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[Preprint]. 2025 Jun 5:2025.06.04.657490. [Version 2] doi: 10.1101/2025.06.04.657490

Figure 3: Caenorhabditis elegans show preference for ingesting M. hiemalis spore and E. coli mixture.

Figure 3:

Preference indexes were calculated from triplicate experiments tested in parallel using 100 nematodes per group. A one-sample t-test was used to compare the preference score to no preference.

(A) A schematic representation of assay plate used for calculating preference indexes. M. hiemalis spores were placed in the top left corner and OP50-1 E. coli was placed in the bottom right corner. 100 C. elegans were introduced in the middle four squares of the plate.

(B) Food preference index of M. hiemalis spores and spore mixture compared to OP50-1. C. elegans showed no preference between fungal spores in water compared to OP50-1 (one-sample t-test, p = 0.2500, n=3). Nematodes showed a significant preference toward a fungal mixture of spores and OP50-1 over OP50-1 alone (one-sample t-test, p = 0.0102, n = 3). Experiment was repeated 3 times independently. Error bars show standard deviation.