Figure 4: M. hiemalis kills Caenorhabditis nematodes with a wide host range.

Survival assays of (A) C. elegans N2, (B) C. japonica DF5081, (C) C. tropicalis JU1373, and (D) C. briggsae SOW21 wild isolate grown on M. hiemalis + OP50 infection plates or OP50 control plates. Infection by M. hiemalis significantly reduced lifespan in each species tested (log-rank test, p < 0.001). Graphs show combined lifespan from three independent biological replicates (n = 20 for each experiment). (E) Table showing median lifespan and percent reduction with Log-rank (Mantel-Cox) tests comparing lifespans of nematodes grown with vs. without M. hiemalis.