Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Jun 11;149(1):61. doi: 10.1007/s00401-025-02894-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — FGFR3 gene body hypomethylation and FGFR3 overexpression are characteristic for CN. A Clustering the methylation values of the FGFR3 locus of primary tumors and control tissue from Capper et al. 2018 revealed severe hypomethylation at the gene body as a distinct feature of CN. However, individual FGFR3 CpG sites, especially three sites denoted as CN specific in a previous study by Lee et al. 2024 showed high variance. FGFR3 methylation levels did not correspond to FGFR3 staining intensity. B-C FGFR3 staining intensity ranged from strong in 30/71 (42%) cases to intermediate in 41/71 (58%) cases. D FGFR3 immunohistochemistry often demonstrated a gradient effect with stronger staining intensity at the periphery with progressively weaker staining towards the center due to a fixation artifact. E Kaplan-Meier estimates did not show a significant PFS difference between cases with strong (+ +) and intermediate (+) staining intensity. F FGFR3 demethylation was consistent across primary and recurrent samples and did not show any association with sample material, the development of a recurrence, or age