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Plasmid DNA amounts for co-transfection in epegRNA architecture screen, related to Step 5
| Plasmid | DNA amount per well of 24-well plate |
|---|---|
| epegRNA-containing pU6-tevopreq1-GG-acceptor | 75 ng |
| pCMV-PEmax-P2A-GFP | 263 ng |
| pEF1a-hMLH1dn | 132 ng |
We typically use the TurboFectin 8.0 transfection reagent (OriGene #TF81005), but an alternative such as Lipofectamine3000 is suitable if it efficiently transfects HEK 293T cells.
