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HEK 293T cell growth media
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM (Gibco #11995-073) | 89% | 44.5 mL |
| FBS (R&D Systems; 50-152-7067) | 10% | 5 mL |
| PenStrep (Gibco #15140122; 100X stock) | 1% | 0.5 mL |
| Total | N/A | 50 mL |
After preparation, growth media should be stored at 4°C for up to 4–6 weeks.
