PCR Cycling Conditions

Steps	Temperature	Time	Cycles
Initial Denaturation	98 °C	3 min	1
Denaturation	98 °C	20 s	30–35 cycles
Annealing	60 °C–62 °C	20 s
Extension	72 °C	30 s
Final extension	72 °C	7 min	1
Hold	4 °C	forever