Fig. 1.

Treatment with A549 and SPC-A-1 CM enhanced endothelial migration. a Confluent monolayer of HUVECs were wounded. Subsequently, the cells were stimulated by A549 and SPC-A-1 CM or treated with 5%FBS medium (Control). Photographs were taken respectively after wounding 0 h and 12 h (40×). b Graphic representation of wound closure was explained in the Materials and methods section. The wound closure following 12 h of control treatment was normalized to 100%, and the wound closure from A549 and SPC-A-1 CM-treated cells was adjusted to the % controls (*P < 0.05 vs. control)