Fig. 3.

A549 CM enhanced endothelial cell survival. HUVECs were exposed to 5%FBS medium (control) and incubated with A549 and SPC-A-1 CM for 24 h. a The cells were harvested and stained with Hoechst. Arrowheads indicated apoptotic cells with fragmented or condensed DNA by fluorescence microscopy (40×). b Representative dot plots of flow cytometric analyses. The cells were stained with annexin V and propidium iodide (PI) and were analyzed by flow cytometry. c Representative histogram of flow cytometric analyses. Bars represent the mean ± SD of three experiments. *P < 0.05 versus control