Fig. 5.

Wortmannin inhibited A549 CM-induced migration and survival of HUVECs. Cells were incubated with control buffer (5%FBS medium + DMSO), A549 CM plus DMSO, or A549 CM plus wortmannin (WT; 100 nmol/L). a Wound-healing assay. Quantification of the endothelial wound repair was done. b XTT assay. The number of viable cells was determined by XTT assay. c Flow cytometry. The apoptotic rates of HUVECs were evaluated by flow cytometry. Bars represent the mean ± SD of three experiments. *P < 0.05 versus control. ^# P < 0.05 versus A549 CM + DMSO