Figure 2.

Effects of reproducibility on lower 95% profile likelihood CLs for γ (solid line, probability that a positive result from a sample is an amplification of sample DNA or of a mixture of sample and contaminant DNA) and γ₂ (dashed line, probability that a positive result from a sample is an amplification of sample DNA only). In this example, a positive result is observed in b_s of 10 sample extracts and none of 10 extraction blanks and 10 PCR blanks. The CIs are the same if we run only 10 blanks of a single kind. The maximum-likelihood estimates of γ and γ₂ and their upper 95% profile CLs are 1, in all cases.