Table 2.
System suitability data for method A in serum.
| Ethylene glycol |
Diethylene glycol |
Triethylene glycol |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| SST Parameter |
Specification | Day 1 |
Day 2 |
Day 3 |
Day 1 |
Day 2 |
Day 3 |
Day 1 |
Day 2 |
Day 3 |
| RT (min) | N/A | 6.83 | 6.83 | 6.83 | 9.76 | 9.76 | 9.76 | 11.83 | 11.83 | 11.83 |
| RSD <2.0% | 0.00 | 0.02 | 0.03 | 0.00 | 0.02 | 0.02 | 0.01 | 0.01 | 0.01 | |
| Area | RSD <5.0% | 0.38 | 3.39 | 1.77 | 0.33 | 3.56 | 1.91 | 1.28 | 3.18 | 2.43 |
| Theoretical Plates | >2000 | 68735 | 80536 | 88853 | 101511 | 104160 | 107720 | 306727 | 337349 | 302414 |
| USP Tailing Factor |
<2.0 | 0.97 | 0.98 | 1.02 | 0.97 | 0.96 | 0.95 | 1.02 | 1.01 | 1.01 |
| RSD <10.0% | 3.15 | 4.84 | 9.58 | 4.43 | 7.88 | 7.90 | 4.45 | 5.95 | 3.56 | |
| Resolution | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 | >2.0 |
| Capacity Factor |
k’ > 3 | 6.18 | 6.18 | 6.17 | 9.25 | 9.25 | 9.25 | 11.42 | 11.42 | 11.42 |
| RSD <2.0% | 0.00 | 0.03 | 0.03 | 0.00 | 0.02 | 0.02 | 0.01 | 0.01 | 0.01 | |
N/A = Not applicable. RT = Retention time. SST = System Suitability Test. AUC = Area Under the Curve. RSD = Relative Standard Deviation. k’ = capacity factor.