Table 4.
Intraday accuracy and intraday precision for method A (serum) and method B (urine).
| Biomatrix and QC level | Ethylene glycol |
Diethylene glycol |
Triethylene glycol |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | ||
| Serum LLQC (2 µg/mL) |
Accuracy | 100.9 | 92.4 | 95.6 | 99.1 | 97.0 | 97.4 | 101.1 | 102.7 | 102.0 |
| %RSD | 3.2 | 4.6 | 5.7 | 0.7 | 1.0 | 1.1 | 4.7 | 5.8 | 3.7 | |
| Serum LQC (4 µg/mL) |
Accuracy | 101.0 | 97.9 | 98.4 | 100.3 | 97.2 | 98.0 | 101.5 | 102.4 | 94.4 |
| %RSD | 1.5 | 1.9 | 1.5 | 0.4 | 0.5 | 0.3 | 4.6 | 7.2 | 4.8 | |
| Serum MQC (7.5 µg/mL) |
Accuracy | 100.6 | 98.6 | 100.0 | 100.1 | 97.8 | 98.8 | 100.7 | 101.0 | 99.6 |
| %RSD | 1.3 | 0.6 | 1.3 | 0.4 | 0.4 | 0.4 | 8.0 | 6.5 | 6.5 | |
| Serum HQC (15 µg/mL) |
Accuracy | 100.8 | 100.6 | 100.5 | 100.2 | 99.0 | 99.5 | 102.4 | 109.3 | 99.1 |
| %RSD | 0.7 | 0.6 | 0.7 | 0.3 | 0.3 | 0.4 | 6.5 | 3.2 | 8.1 | |
| Urine LLQC (2 µg/mL) |
Accuracy | 106.8 | 89.9 | 102.6 | 90.9 | 92.7 | 93.1 | 90.4 | 94.5 | 95.9 |
| %RSD | 8.8 | 8.5 | 11.8 | 2.5 | 6.6 | 8.0 | 3.2 | 5.3 | 4.8 | |
| Urine LQC (4 µg/mL) |
Accuracy | 102.0 | 92.3 | 98.0 | 98.6 | 98.8 | 96.2 | 99.3 | 98.7 | 98.3 |
| %RSD | 6.0 | 2.7 | 4.1 | 5.4 | 3.0 | 10.3 | 1.9 | 0.9 | 2.5 | |
| Urine MQC (7.5 µg/mL) |
Accuracy | 103.0 | 99.6 | 96.7 | 100.7 | 98.9 | 96.4 | 101.2 | 98.9 | 99.5 |
| %RSD | 3.7 | 4.5 | 2.0 | 3.4 | 2.4 | 2.1 | 0.9 | 0.9 | 1.1 | |
| Urine HQC (15 µg/mL) |
Accuracy | 101.0 | 99.3 | 98.0 | 98.8 | 97.3 | 97.0 | 100.3 | 98.4 | 99.0 |
| %RSD | 1.2 | 1.0 | 0.7 | 0.8 | 1.6 | 1.9 | 0.8 | 0.8 | 1.0 | |
LLQC = Lower Limit Quality Control. LQC = Low Quality Control. MQC = Middle Quality Control. HQC = high Quality Control. RSD = Relative Standard Deviation.