Table 4.
The levels of sialyltransferase activities in human gastric carcinoma
| Tissue specimen | Sialyltransferase activity: incorporation of [9−3H]NeuAc(CPM × 10−3) catalyzed by 1 mg protein of the tissue extract | ||
|---|---|---|---|
| ST3(O) | ST3(N) | ST6(N) | |
| NS7323 | 1.9 | 0.4 | 0 |
| GC7323 | 326.8 ↑(172.0) | 7.1 ↑(4.3) | 0 |
| NS7741 | 184.5 | 2.1 | 0.4 |
| GC7741 | 53.8 ↓ (0.3) | 3.1 ↑(1.5) | 32.9 ↑(82.2) |
| NS7405 | 54.4 | 1.9 | 15.4 |
| GC7405 | 145.1 ↑(2.7) | 4.0 ↑(2.1) | 8.3 ↓(0.5) |
| NS7570 | 234.2 | 1.1 | 16.2 |
| GC7570 | 155.2 ↓(0.7) | 1.6 ↑(1.5) | 6.4 ↓(0.4) |
| NS9529 | 104.2 | 1.7 | 48.3 |
| GC9529 | 240.3 ↑(2.3) | 2.3 ↑(1.4) | 11.9 ↓(0.2) |
| NS11681 | 306.5 | 2.6 | 8.3 |
| GC11681 | 245.1 ↓(0.8) | 2.2 ↓(0.8) | 38.4 ↑(4.6) |
| NS11868 | 180.4 | 0.7 | 24.5 |
| GC11868 | 309.0 ↑(1.7) | 2.5 ↑(3.6) | 25.2 ↑(1.0) |
| NS11954 | 15.6 | 0 | 0 |
| GC11954 | 122.7 ↑(7.9) | 0 | 7.3 ↑ |
| NS11847 | 379.7 | 0.6 | 40.5 |
| GC11847 | 83.3 ↓(0.5) | 4.2 ↑(7.0) | 65.0 ↑(1.6) |
| NS12015 | 148.6 | 3.6 | 50.1 |
| GC12015 | 308.2 ↑(2.1) | 3.8 ↑(1.1) | 41.5 ↓(0.8) |
| GC7532 | 114.0 | 1.1 | 8.7 |
| GC11577 | 91.9 | 2.7 | 8.2 |
Values in parentheses indicate the fold of enzyme activity in tumor specimen as compared to that of corresponding normal tissue of the patient
Acceptors used: ST3(O): 3-O-MeGalβ1,4GlcNAcβ1,6(Galβ1,3)GalNAcα-O-Bn; ST3(N): 2-O-MeGalβ1,3GlcNAcβ-O-Bn; ST6(N): the values obtained by using the acceptor Galβ1,4GlcNAcβ1,6(3-O-MeGalβ1,3)GalNAcα-O-Bn were corrected by subtracting the values obtained by using Galβ1,3GlcNAcβ-O-Bn, which is an acceptor for ST3(N) and also to some extent active with ST3(O)
NS Normal stomach, GC gastric carcinoma
↑ Increase, ↓ decrease