Figure 6.

The expression of TNF-α, IL-2, and IL-4 in BMSCs treated with different doses of sodium butyrate. GC -: DXM 0 mM; GC +: DXM 100 mM; SCFA -: sodium butyrate 0 mM; SCFA +: sodium butyrate 1 mM; SCFA ++: sodium butyrate 5 mM. (A–C) Immunofluorescence staining of TNF-α (A), IL-2(B) and IL-4(C) in BMSCs. (D to G) Western blot (D) and quantitative analysis of TNF-α (E), IL-2(F), and IL-4(G) in BMSCs. ∗p < 0.05. BMSCs, bone marrow mesenchymal stem cells; GC, glucocorticoids; IL, interleukin; SCFA, short-chain fatty acids; TNF-α, tumor necrosis factor-alpha.