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. 2005 Sep 15;33(16):5243–5249. doi: 10.1093/nar/gki835

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Unequal SCE assay. (A) The his3 unequal SCE substrate. The his3-Δ3′ construct is marked with a tail, and the his3-Δ5′ construct is marked with an arrowhead. The shaded region indicates the region of homology between the two truncated his3 fragments. A wild-type HIS3 gene can form by unequal exchange (B) or by a non-reciprocal gene conversion event (C).