Table 1.
Parameters for the quantitative liquid chromatography-tandem mass spectrometry detection of the cocktail substrates
| Analyte | MRM (m/z) | CE [Volt] | RT [Min] | Internal Standard |
IS MRM (m/z) | CE [Volt] | RT [Min] |
|---|---|---|---|---|---|---|---|
| Fenoterol | 304.3/107.1 | 50 | 5.45 | Fenoterol-d6 | 310.3/141.2 | 25 | 5.42 |
| Salbutamol | 240.3/148.1 | 25 | 3.88 | ||||
| Sumatriptan | 296.2/58.1 | 43 | 5.03 | Sumatriptan-d6 | 302.4/64.2 | 45 | 5.02 |
| Zolmitriptan | 288.5/58.1 | 50 | 5.37 | ||||
| Ipratropium | 332.3/166.3 | 35 | 6.32 | ||||
| Methylnaltrexone | 356.4/284.2 | 35 | 4.91 | ||||
| Metformin | 129.9/71 | 32 | 1.68 | ||||
| Trospium | 392.3/164.3 | 43 | 7.65 | Trospium-d8 | 401.4/172.3 | 44 | 7.63 |
| L-carnitine-d9 | 171.2/103.2 | 26 | 1.61 | Isobutyryl-L-carnitine-d6 | 238.40/58.1 | 27 | 3.64 |
| L-ergothioneine | 230.3/127.2 | 27 | 2.02 |
CE, collision energy; MRM, multiple reaction monitoring; RT, retention time.