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. 2025 May 30;14:e64379. doi: 10.2196/64379

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©Vitasari Indriani, Teguh Triyono, Budi Mulyono. Originally published in JMIR Research Protocols (https://www.researchprotocols.org), 30.05.2025.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on https://www.researchprotocols.org, as well as this copyright and license information must be included.

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Flowchart of β-thalassemia major participants, eligible participants will be enrolled, and peripheral blood and urine samples will be collected before and after transfusion. Required clinical data will be collected from patients’ medical records. LDH: lactate dehydrogenase.