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. 2025 May 17;32:101878. doi: 10.1016/j.mtbio.2025.101878

Fig. 1.

Fig. 1

Impact of mPEG-CDM modification on sEV properties. (a) Chemical structure of mPEG-CDM. (b) UV–Vis spectra of CDM and mPEG-CDM. (c) Coomassie Brilliant Blue staining of BSA modified with mPEG-CDM at concentrations of 0, 1, 2, 4, and 8 mg/mL. (d) Coomassie Brilliant Blue staining of BSA alone and BSA modified with mPEG-CDM under pH 7.4 and pH 6.0 conditions. (e) Western blot analysis of CD9 in GsEVs, IsEVs, and FsEVs before and after mPEG-CDM modification under pH 7.4 and pH 6.0 conditions. (f) Representative TEM images of GsEVs, IsEVs, and FsEVs before and after mPEG-CDM modification. Scale bar: 100 nm. (g) Zeta potential of GsEVs, IsEVs, and FsEVs before and after mPEG-CDM modification. (h) Particle size distribution of GsEVs, IsEVs, and FsEVs before and after mPEG-CDM modification. Data are shown as mean ± SD (n = 5). ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)