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. 2025 May 17;32:101878. doi: 10.1016/j.mtbio.2025.101878

Fig. 2.

Fig. 2

Cellular uptake and pH-responsive behavior of mPEG-CDM-modified sEVs. (a) Representative fluorescence images of RAW 264.7 cells with unmodified and mPEG-CDM-modified GsEVs, IsEVs, and FsEVs at pH 7.4. (b) Flow cytometry histograms and corresponding statistical analysis of MFI for RAW 264.7 cells with unmodified or mPEG-CDM-modified sEVs at pH 7.4. (c) Representative fluorescence images of RAW 264.7 cells with acid-pretreated and untreated mPEG-CDM-modified GsEVs, IsEVs, and FsEVs. (d) Flow cytometry histograms and corresponding statistical analysis of MFI for RAW 264.7 cells with acid-pretreated and untreated mPEG-CDM-modified sEVs. (e) Representative fluorescence images of GL261 cells with mPEG-CDM-GsEVs at pH 7.4, 6.5, and 6.0 for 2, 6, and 12 h. (f) Flow cytometry histograms of GL261 cells with mPEG-CDM-GsEVs at pH 7.4, 6.5, and 6.0 for 2, 6, and 12 h. (g) Statistical analysis of MFI from (f). (h) Representative fluorescence images of bEnd.3 cells with mPEG-CDM-IsEVs at pH 7.4, 6.5, and 6.0 for 2, 6, and 12 h. (i) Flow cytometry histograms of bEnd.3 cells with mPEG-CDM-IsEVs at pH 7.4, 6.5, and 6.0 for 2, 6, and 12 h. (j) Statistical analysis of MFI from (i). Data are shown as mean ± SD (n = 5). ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant. DiD (red)-labeled sEVs; DAPI (blue)-labeled nuclei. Scale bar: 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)