Fig. 5.

Claramine A1 and spermine treatments decreased P. aeruginosa H103 membrane fluidity. Membrane fluidity assessment by fluorescence anisotropy using 1,6-diphenyl-1,3,5-hexatriene (DPH) probe in P. aeruginosa H103 upon treatment with ultrapure Milli-Q® water (red bar), squalamine (blue bar), claramine A1 (orange bar), spermidine (light blue bar), or spermine (pink bar). Each experiment was assayed at least four times independently. Statistics were performed by ordinary one-way ANOVA followed by Tukey's multiple-comparison test. Significance was considered at ∗∗∗∗, p < 0.0001; ∗∗, p = 0.001–0.01; ns (not significant), p > 0.05.