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. 2025 Apr 6;44(25):2091–2102. doi: 10.1038/s41388-025-03365-5

Fig. 1. In vitro cultured KPN organoids and tumoroids display differential WNT activity.

Fig. 1

a Schematic overview of the KPN mouse model. b Representative images of KPN organoids and tumoroids cultured 3D in Matrigel. Scale bar, 600 μm. c Volcano plot for differentially expressed genes (DEG) in tumoroids and organoids. Four tumoroid lines were derived from four different KPN mice (N = 4). Two organoid lines were derived from two different KPN mice and two independent experiments were performed (N = 4). Pink denotes downregulated genes in tumoroids with log2 fold change >-1, blue denotes upregulated genes in tumoroids with log2 fold change >1. d Wiki Pathways gene set enrichment (WP428) of 1000 most upregulated genes (fold change >1, p < 0.05) in tumoroids (bulk). e IHC staining for β-catenin protein or Notum in situ hybridization (ISH) on organoids and tumoroids. Scale bar, 50 μm. Scale bar zoom panel, 10 μm. f Flow Cytometry for WNT reporter activity measured on 640 nm laser. g Geometrical mean of WNT reporter fluorescence relative to untransduced parental cells lacking the WNT reporter. Dots represent N = 3 independent experiments. Data are mean±s.e.m and analyzed with unpaired two-tailed Student’s t test.