Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Apr 6;44(25):2091–2102. doi: 10.1038/s41388-025-03365-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 3 — a Schematic overview of in vivo experiment with intracolonic injection of KPN tumoroids and organoids. b Representative endoscopic images of colonic tumor growth over time till endpoint was reached ( > 80% bowel obstruction). Arrow indicates tumor. c Kaplan-Meier curve of colon tumor-free survival. Organoids, n = 6; tumoroids, n = 8. Data analyzed by log-rank test. d Proportion of tumor growth in colon analyzed at human/clinical endpoint. e Proportion of presence of metastasis at human/clinical endpoint. f Representative image of intestine with tumor at clinical endpoint ( > 80% bowel obstruction). g Representative Notum ISH and β-catenin/CDX2 IHC staining on colonic tumors derived from organoid and tumoroids. Insets represent zoomed-in images of dashed areas. Scale bar, 100 μm. Scale bar zoom panel, 10 μm. h Location of metastases originating from tumor grown from intracolonic tumoroid transplantation. i H&E staining of liver with metastasis derived from intracolonic tumoroid transplantation. Scale bar, 100 μm. j Representative Lgr5 ISH on tumors derived from intracolonic tumoroid / organoid transplantation and liver metastasis derived from intracolonic tumoroid transplantation. Insets represent zoomed-in images of dashed areas. Scale bar, 100 μm. Scale bar zoom panel, 20 μm. k Schematic overview of in vivo experiment with intraperitoneal (IP) injection and PCI-scoring areas. l H&E staining of IP tumor grown from IP tumoroid transplantation. Scale bar, 400 μm. m PCI score including tumor size and quantity. Organoids N = 6, tumoroids N = 5. Data mean ± s.e.m;, analyzed with unpaired two-tailed Student’s t test. n Notum ISH and β-catenin/CDX2 IHC staining on IP tumors derived from IP transplantations of organoids or tumoroids. Insets represent zoomed-in images of dashed areas. Scale bar, 100 μm. Scale bar zoom panel, 10 μm.