Table 2. Effects of P18, P24, and Ap2A on the [Ca2+]i and on the content of thapsigargin-sensitive Ca2+ stores in CD38+/- HeLa cells.
| [Cai2+], nM
|
||||
|---|---|---|---|---|
| Cells | Control | P18 | P24 | Ap2A |
| HeLa CD38+ | 37 | 16 | 57 | 79 |
| 254 | 123 | 203 | 184 | |
| HeLa CD38– | 23 | 12 | 44 | 35 |
| 470 | 366 | 290 | 398 | |
Basal [Ca2+]i and thapsigargin-induced Ca2+ release (bold numbers) were measured on FURA 2-loaded CD38+ and CD38– HeLa incubated in the absence (control) or in the presence of 1 M dinucleotides for 18 hr. During this time, partial (≤30%) dinucleotide degradation occurred in CD38+ cell cultures, with production of Ade and hypoxanthine (see Table 3). Mean results from at least eight different determinations are shown; SD ≤ 15% of the mean value.