|
| Antibodies |
|
| Chicken anti-GFP |
Abcam |
Cat#ab13970; RRID:AB_300798 |
| Mouse anti-Draper |
Developmental Studies Hybridoma Bank (DSHB) |
RRID: AB_2618105 (clone 5D14) |
| Mouse anti-Draper |
DSHB |
RRID: AB_2618106 (clone 8A1) |
| Rabbit anti-Apo-II |
Rajan Lab |
Brent and Rajan114
|
| Rabbit anti-Dilp5 |
Rajan Lab |
Brent and Rajan114
|
| Rabbit mito-mCherry |
Abcam |
Cat#ab167453, RRID: AB_2571870 |
| Donkey anti-Chicken Alexa 488 |
Jackson ImmunoResearch |
Cat#703-545-155; RRID: AB_2340375 |
| Donkey anti-Rabbit Alexa 594 |
Jackson ImmunoResearch |
Cat#711-585-152; RRID: AB_2340621 |
| Donkey anti-Rabbit Alexa 488 |
Jackson ImmunoResearch |
Cat#711-545-152; RRID: AB_2313584 |
| Donkey anti-Mouse Alexa 594 |
Jackson ImmunoResearch |
Cat#715-585-150; RRID: AB_2340854 |
| Mouse anti-Tubulin |
Sigma-Aldrich |
Cat#T5168; RRID: AB_477579 |
|
| Chemicals, peptides and recombinant proteins |
|
| Pyruvate |
Agilent |
Cat#103578-100 |
| HALT Protease Inhibitor Cocktail |
ThermoFisher |
Cat#87786 |
| 5x RIPA Buffer |
ThermoFisher |
Cat#J62524.AD |
| Laemmli Sample Buffer |
Bio-Rad |
Cat#161047 |
| TCEP Bond Breaker |
Thermo Scientific |
Cat#77720 |
| Precision Plus Protein™ Dual Color Standards |
Bio-Rad |
Cat#1610374 |
| Starting Block™ Blocking Buffer |
Thermo Scientific |
Cat#37538 |
| Enhanced Chemiluminescence (ECL) detection system |
Abcam |
Cat#AB65623 |
| TriReagent |
Sigma-Aldrich |
Cat#T9424 |
| iScript RT supermix |
Bio-Rad |
Cat#1708841 |
| ssoAdvanced SYBR green master mix |
Bio-Rad |
Cat#1725270 |
|
| Critical commercial assays |
|
| Free Fatty Acid Assay Kit |
Sigma-Aldrich |
Cat#MAK466 |
| Pierce™ BCA Protein Assay Kit |
Thermo Scientific |
Cat#23228 |
| Direct-zol RNA microprep kit |
Zymo Research |
Cat#R2060 |
|
| Deposited data |
|
| Whole Fly Lysate Aqueous Metabolomic datasets for ND, HSD (7 and 14 days) |
Figshare |
https://doi.org/10.6084/m9.figshare.28765562.v1
|
| Hemolymph Aqueous Metabolomic datasets for ND, HSD (7 and 14 days) |
Figshare |
https://doi.org/10.6084/m9.figshare.28765067.v2
|
| Hemolymph Lipidomics datasets for ND, HSD (14 days) |
Figshare |
https://doi.org/10.6084/m9.figshare.28765064.v1
|
|
| Experimental models: Organisms/strains |
|
| UAS-Plin1:GFP |
Kuhnlein Lab |
Mathias115
|
| Ldh-GFP |
Tennessen Lab |
Brent and Rajan114
|
| ppl-Gal4 |
Leopold Lab |
N/A |
| Lpp-Gal4 |
Eaton Lab |
N/A |
| UAS-mitoTIMER |
Bloomington Drosophila Stock Center (BDSC) |
BDSC#57323 |
| UAS-mitoPyronicSF |
BDSC |
BDSC#94536 |
| UAS-Fis1-RNAi |
BDSC |
BDSC#63027 |
| UAS-SOD2-RNAi |
BDSC |
BDSC#36871 |
| UAS-Apolpp-RNAi |
BDSC |
BDSC#33388 |
| UAS-LpR1-RNAi |
BDSC |
BDSC#27249 |
| UAS-LpR2-RNAi |
BDSC |
BDSC#31150 |
| UAS-Catalase |
BDSC |
BDSC#24621 |
| UAS-whd-RNAi |
BDSC |
BDSC#34066 |
| UAS-Acat1-RNAi |
BDSC |
BDSC#51785 |
| Or22a-mCD8GFP |
BDSC |
BDSC#52620 |
| Ensheathing glia-Gal4 |
BDSC |
BDSC#39157 |
| UAS-Luc-RNAi |
BDSC |
BDSC#31603 |
| UAS-bmm-RNAi |
Vienna Drosophila Resource Center (VDRC) |
VDRC#37880 |
| upd2-del |
Zeidler Lab |
N/A |
|
| Oligonucleotides |
|
| Primers |
See Table S3
|
See Table S3
|
|
| Software and algorithms |
|
| ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
| GraphPad PRISM |
GraphPad Software Incorporated |
https://www.graphpad.com/scientific-software/prism/
|
| AB Sciex Analyst 1.6.3 |
AB Sciex |
https://sciex.com/products/software/analyst-software
|
| AB Sciex MultiQuant 3.0.3 |
AB Sciex |
https://sciex.com/products/software/multiquant-software
|
|
| Other |
|
| Zeiss LSM 800 Confocal Microscope |
Zeiss |
N/A |
| Leica Stellaris Confocal Microscope |
Leica Microsystems |
N/A |
