2. Activation Energies (E a) and Binding Energies (BE) Calculated for the Enzyme Complexed with Substrates Containing Different R Groups .

| Substrate | R2 | R3 | Ea(expt.) | Ea(Calc.) |
|---|---|---|---|---|
| 1 | H | H | 15.80 | 16.5 |
| 2 | CH3 | H | 15.83 | 15.6 |
| 3 | NH2 | H | 18.90 | 19.6 |
| 4 | Phenyl | H | 18.99 | 28.9 |
| 5 | H | CH3 | 18.73 | 22.9 |
Values are in kcal/mol.