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. 2025 Jun 16;19(2):e70023. doi: 10.1002/ccs3.70023

FIGURE 3.

FIGURE 3

Angio‐associated migratory cell protein (AAMP) regulates colorectal cancer cell proliferation and colony formation in vitro through knockdown or overexpression. (A) Western blot analysis showing AAMP knockdown efficiency in HCT‐15 and HT55 cells. (B) Crystal violet staining assay assessing the impact of AAMP knockdown on colony formation in HCT‐15 and HT55 cells. (C) Western blot analysis showing AAMP overexpression efficiency in HCT‐15 and HT55 cells. (D) Crystal violet staining assay evaluating the effect of AAMP overexpression on colony formation in HCT‐15 and HT55 cells. (E) 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay measuring the effect of AAMP knockdown on cell proliferation in HCT‐15 and HT55 cells with absorbance measured at 570 nm. (F) Tumor imaging and size comparison from mice implanted with scramble control or AAMP knockdown HT55 cells (n = 6). (G) MTT assay assessing the effect of AAMP overexpression on cell proliferation in HCT‐15 and HT55 cells with absorbance measured at 570 nm. (H) Tumor imaging and size comparison from mice implanted with vector control or AAMP overexpression HT55 cells (n = 6). Data are presented as means ± SD with three independent experiments performed. **p < 0.01, ***p < 0.001, ****p < 0.0001.