Fig. 6.

HSP70 loss of function impairs coronary artery angiogenesis in vitro (a) HCAECs were cultured under basal conditions and then plated in Matrigel in the presence of the inhibitors VER-155008 (50 µM) or YM-1 (40 µM). After 5 h of incubation, images were acquired. Representative images of triplicate independent experiments are shown. (b) Representative image of angiogenesis analysis with a map of selected parameters. (c) Quantification of the total branching length (left) and number of master segments (right). Each point represents repeated measurements taken in three independent experiments (N) for each treatment (symbol). The insets show the results of two-way ANOVA with Tukey’s post hoc test; ****P < 0.0001 indicates significant differences among means. Abbreviations used: HCAEC, human coronary artery endothelial cell; HUVEC, human vein.