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. 2025 May 22;99(6):e00666-25. doi: 10.1128/jvi.00666-25

Fig 1.

Maternal viremia varies by strain and dose. Embryo viability declines with 10^5 PFU ZIKV-MEX and 10^3 PFU ZIKV-BRA between E11.5 and E4.5. Resorption rate and reduced crown-to-rump length reflect infection severity across gestation ages.

ZIKV strain phenotypic heterogeneity is present across gestation. (A) Time-mated Ifnar1−/− dams were inoculated with 103 PFU ZIKV-MEX, 105 PFU ZIKV-MEX, or 103 PFU ZIKV-BRA on E7.5. Maternal infection was assayed by plaque assay on 2, 4, and 7 days post-inoculation, and significance was determined by two-way ANOVA with Tukey’s multiple comparisons. (B) Rate of normal (black) vs resorbed (colored) fetuses at E11.5, E14.5, and E17.5 after maternal infection at E7.5. Data are presented as the percentage of n = 26–83 total fetuses (from 3 to 10 dams per treatment group). Significance was determined by Fisher’s exact test. (C) Pregnancy outcomes of individual animals in each treatment group. Data are presented as percentage of fetuses resorbed in each pregnancy. (D) Crown-to-rump length measurements in millimeters of morphologically normal fetuses at E11.5, E14.5, and E17.5 using ImageJ software. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons. Gray indicates historical data from reference 18. Significance annotations: ****, P  ≤  0.0001; *, P  ≤  0.05.