REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Cell lysis buffer	Thermo Fisher Scientific, Waltham, MA	FNN0021
Phenylmethanesulfonyl fluoride (PMSF)	Sigma–Aldrich, Oakville, ON	P7626
Protease inhibitor	Sigma–Aldrich, Oakville, ON	P2714
β-secretase substrate IV, fluorogenic	Sigma–Aldrich, Oakville, ON	565758
β-secretase inhibitor	Sigma–Aldrich, Oakville, ON	S4562
Active β-secretase	Sigma–Aldrich, Oakville, ON	S4195
Sodium acetate	Sigma–Aldrich, Oakville, ON	S2889
Software and algorithms
SoftMax Pro 7 software	Molecular Devices	N/A
Biological samples
Human postmortem brain homogenate, male (78.6 ± 8.1 years of age) and female (79.4±7.8 years of age) non-demented controls with no AD diagnosis, and male (82.4±5.9 years) and female (81.2±7.0 years of age) samples with AD status	The Douglas Bell Canada Brain Bank (Montreal, Quebec, Canada; http://douglasbrainbank.ca)	N/A
Experimental models: Cell lines
SH-SY5Y neuroblastoma cell line	ATCC	N/A
Experimental models: Organisms/strains
Murine (male, C57BL/6, 3–4 months) Prefrontal cortex homogenate	The Jackson Laboratory	N/A
Other
All black 96-well plates	Thermo Fisher Scientific, Waltham, MA	7805
Eppendorf repeater pipette	Sigma–Aldrich, Oakville, ON	EP4982000322
SpectraMax M2 microplate reader	Molecular Devices, San Jose, CA	https://www.moleculardevices.com/products/microplate-readers/
1.5 mL microcentrifuge tubes	Thermo Fisher Scientific, Waltham, MA	14-222-158