Table 2.
Comparison of acquisition modes (AcquireX, DDA, and DIA) based on total detected features and identification performance. Values represent the mean and coefficient of variation (CV, %) over three weekly measurements as presented in fig. 2 (each weekly measurement is derived from triplicate injections of unspiked TLE samples). MS1-based identifications were performed using mass list and ChemSpider, while MS2-based identifications were matched using mzCloud. All values are after background feature removal and were acquired in negative-ion mode.
| Total detected | MS1 -match | MS2 -match | ||
|---|---|---|---|---|
| AcquireX | Mean | 654 | 474 | 50 |
| CV / % | 15 | 18 | 4 | |
| DDA | Mean | 854 | 579 | 43 |
| CV / % | 17 | 21 | 19 | |
| DIA | Mean | 1036 | 692 | 52 |
| CV / % | 10 | 9 | 6 |