Table 1.

Lineage cocktail master mix preparation

Reagent	Concentration	Volume (μL)
Biotin-Gr1/Ly-6G	0.5 mg/ml	100
Biotin-Ter119	0.5 mg/ml	100
Biotin-CD4	0.5 mg/ml	25
Biotin-CD8	0.5 mg/ml	25
Biotin-CD45R/B220	0.5 mg/ml	50
Biotin-CD127	0.5 mg/ml	50
Sterile PBS	N/A	350
Total	N/A	700

Components and volumes for preparing the lineage antibody cocktail used for marking and excluding differentiated hematopoietic cells in flow cytometry analysis.