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. 2025 May 29;33:101917. doi: 10.1016/j.mtbio.2025.101917

Fig. 3.

Fig. 3

Evaluation of si-Cx43-NPs@Avastin-hydrogel in hREC cells and its anti-angiogenic effects in vitro (A) Schematic illustration of cell experiments. (B) CCK-8 assay assessing cell viability after treatment with nanoparticles and hydrogels. (C) Schematic illustration of cell experiments under HG conditions. (D) qRT-PCR analysis of Cx43 and VEGF mRNA levels in hREC cells treated with nanoparticles or hydrogels under HG conditions. (E) Immunoblotting for Cx43 and VEGF protein levels in treated hREC cells. (F) Immunofluorescent staining (IF staining) detectsing Ki67 for cell proliferation. (G) Tube formation assay evaluating the anti-angiogenic effects of hydrogels in vitro. ∗∗p < 0.01, vs control; ##p < 0.01 vs si-Cx43-NPs@Avastin-hydrogel.