Table 2.
lncRNA45-protein interactions observed in S1m in vitro pull-down.
| lncRNA45 | ||||||
|---|---|---|---|---|---|---|
| Code | Descriptiona | Accession Numberb | GeneID M2904c | GeneID M2903c | Sized | pValuee |
| Lb1 | 40S ribosomal protein S9, putative | A4H519 | LbrM.07.0750 | LBRM2903_070014500 | 22 kDa | 95 % (0.0029) |
| Lb2 | XRN 5′-3′ exonuclease N-terminus, putative | A4H8G4 | LbrM.16.0400 | LBRM2903_160009500 | 108 kDa | 95 % (0.011) |
| Lb3 | Lupus La protein homolog, putative | A4HBQ1 | LbrM.21.0600 | LBRM2903_210010600 | 37 kDa | 95 % (0.029) |
| Lb4 | hypothetical protein, conserved | A4HGJ6 | LbrM.28.1600 | LBRM2903_280022100 | 80 kDa | 95 % (0.014) |
| Lb5 | Nop53 (60S ribosomal biogenesis), putative | A4HMS1 | LbrM.34.2420 | LBRM2903_340031800 | 38 kDa | 95 % (0.0080) |
| Lb6 | RNA-binding protein, putative | A4HI65 | LbrM.30.1230 | LBRM2903_300017800 | 67 kDa | 95 % (0.014) |
| Lb7 | tRNA (Uracil-5-)-methyltransferase, putative | E9AIL9 | LbrM.20.5870 | LBRM2903_200073900 | 66 kDa | 95 % (0.047) |
| Lb8 | DNA-directed RNA polymerase II subunit 3, putative | A4HH22 | LbrM.29.0150 | LBRM2903_290006800 | 37 kDa | 95 % (0.037) |
| Lb9 | dihydrouridine synthase (Dus), putative | A4H8X8 | LbrM.17.0330 | LBRM2903_170009100 | 66 kDa | 95 % (0.037) |
| Lb10 | Mitochondrial small ribosomal subunit Rsm22, putative | A4HD89 | LbrM.24.0330 | LBRM2903_240008300 | 110 kDa | 95 % (0.031) |
| Lb11 | ISWI complex protein | A4HEU2 | LbrM.26.0880 | LBRM2903_260013600 | 80 kDa | 95 % (0.026) |
| Lb12 | kinetoplast-associated protein, putative | A4HQA1 | LbrM.35.6130 | LBRM2903_350072700 | 14 kDa | 95 % (0.041) |
| Lb13 | acetyl-CoA carboxylase | A4HJT6 | LbrM.31.3340 | LBRM2903_310043300 | 241 kDa | 95 % (0.0053) |
| Lb14 | N-terminal region of Chorein, a TM vesicle-mediated sorter, putative | A4H8K0 | LbrM.16.0770 | LBRM2903_160014400 | 604 kDa | 95 % (0.049) |
| Lb15 | Symplekin tight junction protein C terminal, putative | A4HJL9 | LbrM.31.2640 | LBRM2903_310035900 | 165 kDa | 95 % (0.0028) |
| Lb16 | replication factor C, subunit 3, putative | A4HMZ2 | LbrM.34.3160 | LBRM2903_340040300 | 40 kDa | 95 % (0.011) |
| Lb17 | hypothetical protein, conserved | A4HHV4 | LbrM.30.0100 | LBRM2903_300006000 | 24 kDa | 95 % (0.042) |
| Lb18 | hypothetical protein, conserved | A4HPR3 | LbrM.35.4190 | LBRM2903_350052100 | 51 kDa | 95 % (0.0021) |
Bold labelling refers to proteins specifically binding to lncRNA45WT.
a
Protein description based on TritrypDB annotation.
b
Uniprot accession number.
c
Gene identification in TritrypDB.
d
Protein size in kilodalton (kDa).
e
pValue and % of confidence obtained in the statistical analysis. One-Way ANOVA was used to compare S1m results of the negative control, the lncRNA45WT and lncRNA45MUT pull-downs. Unpaired T-test was used to compare S1m results of the negative control with the lncRNA45 pull-down. The differences were considered significant when p < 0.05.