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. 2025 Jun 5;16:1591835. doi: 10.3389/fneur.2025.1591835

Figure 1.

Figure 1

Detection of anti-GFAP antibody by CBA (A1–F1) and TBA (A2–D2) in patient 14. (A1–F1) GFAP-IgG were tested by a CBA using HEK293 cells transiently cotransfected with full-length human GFAP and pLV-mCherry-N. The patient’s IgG bound to GFAP-transfected cells and showed green fluorescence as a positive control. (A1–C1) GFAP-IgG in serum were tested. (D1–F1) GFAP-IgG in CSF were tested. (A2–D2) TBA was performed with an indirect immunofluorescence assay using standard monkey hippocampus and cerebellum tissue. (A2–B2) Serum specific IgG binds to the white matter astrocytes in monkey cerebellar tissue, consistent with GFAP distribution. (C2–D2) CSF specific IgG binds to the white matter astrocytes in monkey cerebellar tissue, consistent with GFAP distribution.